Journal: Brain research bulletin

Article Title: The decrease of GluN2B and its phosphorylation at Tyr-1336 in extrasynaptic subunits is associated with neuroprotection induced by hypoxia preconditioning.

doi: 10.1016/j.brainresbull.2025.111400

Figure Lengend Snippet: Fig. 4. Effects of hypoxia preconditioning on the protein level of Cleaved Caspase-3 and α-spectrin protein expression in the mouse hippocampus. A: Representative Western blotting results showing Caspase-3 and α-spectrin; B: Semi-quantitative analysis showing the protein changes in 120 kDa α-spectrin levels(n = 5 per group). C: Semi-quantitative analysis showing the protein changes in Cleaved Caspase-3 levels(n = 5 per group). * p﹤0.05 versus N group; # p﹤0.05 versus H group. N: Normoxia; H: Hypoxia; HPC: Hypoxia Preconditioning.

Article Snippet: The membranes were incubated overnight with the following primary antibodies: (GluN2B (Cell Signaling Technology, #4207), p-Y1336 GluN2B (Phosphosolutions, #p1516–1336), p-Y1252 GluN2B (Phosphosolutions, #p1516–1252), spectrin (Santa Cruz Biotechnology, sc-48382), caspase-3(Cell Signaling Technology, #9662), β-actin (Santa Cruz Biotechnology, sc-47778), PSD95 antibody (Cell Signaling Technology, #3450),GluN1 antibody (Cell Signaling Technology, #5704),GluN2A antibody (Cell Signaling Technology, #4205),EEA1 antibody (Cell Signaling Technology, #2411),Rab11 antibody (Cell Signaling Technology, #5589), p97 ATPase antibody (Cell Signaling Technology, #2649)), and then incubated with secondary antibodies for 1 h. The blots were detected using ECL ultrasensitive luminescence solution (Zhang et al., 2019).

Techniques: Expressing, Western Blot

Journal: Brain research bulletin

Article Title: The decrease of GluN2B and its phosphorylation at Tyr-1336 in extrasynaptic subunits is associated with neuroprotection induced by hypoxia preconditioning.

doi: 10.1016/j.brainresbull.2025.111400

Figure Lengend Snippet: Fig. 6. Effects of hypoxia preconditioning on the protein expression of Cleaved Caspase-3 and α-spectrin in HT22 cells. A: Representative Western blotting results showing cleaved Caspase-3 and α-spectrin; B: Semi-quantitative analysis showing the protein changes in 120 kDa α-spectrin levels (n = 5 per group). C: Semi- quantitative analysis showing the protein changes in Cleaved Caspase-3 levels(n = 5 per group). * p﹤0.05 versus N group; # p﹤0.05 versus H group. N: Normoxia; H: Hypoxia; HPC: Hypoxia Preconditioning.

Article Snippet: The membranes were incubated overnight with the following primary antibodies: (GluN2B (Cell Signaling Technology, #4207), p-Y1336 GluN2B (Phosphosolutions, #p1516–1336), p-Y1252 GluN2B (Phosphosolutions, #p1516–1252), spectrin (Santa Cruz Biotechnology, sc-48382), caspase-3(Cell Signaling Technology, #9662), β-actin (Santa Cruz Biotechnology, sc-47778), PSD95 antibody (Cell Signaling Technology, #3450),GluN1 antibody (Cell Signaling Technology, #5704),GluN2A antibody (Cell Signaling Technology, #4205),EEA1 antibody (Cell Signaling Technology, #2411),Rab11 antibody (Cell Signaling Technology, #5589), p97 ATPase antibody (Cell Signaling Technology, #2649)), and then incubated with secondary antibodies for 1 h. The blots were detected using ECL ultrasensitive luminescence solution (Zhang et al., 2019).

Techniques: Expressing, Western Blot

Journal: bioRxiv

Article Title: Lipid scrambling via TMEM16F mediates the formation and release of apoptotic vesicles

doi: 10.1101/2025.05.14.654002

Figure Lengend Snippet: A) WT/YA/YQ TMEM16F-mCherry and LifeAct-GFP-expressing HeLa cells were labelled live for exposed PS AnV. B) Analysis/quantification of F-actin in induced cells. C) Western blotting for α2 spectrin in HeLa cells. Intact spectrin and products of its degradation indicated. D) WT/YA/YQ inducible HeLa cells treated with blebbistatin, a caspase inhibitor (Q-VD-OPh) or calpeptin labelled with AnV. E) Vesicle quantification upon blebbistatin treatment of inducible HeLa cells. F) Vesicle quantification in inducible Jurkat cells. G) Vesicle quantification upon caspase inhibitor treatment of inducible HeLa cells. H) Vesicle quantification upon caspase inhibitor treatment of inducible Jurkat cells. I) Vesicle quantification in calpeptin treated inducible HeLa cells. J) Vesicle quantification in calpeptin treated of inducible Jurkat cells. All scale bars: 20 μm

Article Snippet: Primary antibodies used were: 1:1000 spectrin α2 (Santa Cruz, sc-48382), 0.4 μg/mL GAPDH (Sigma; Cat# MAB374).

Techniques: Expressing, Western Blot